The measurement of intracellular reactive oxygen species (ROS) levels provides critical insights into cell health and pathophysiology. Here, we present a semi-quantitative, plate reader-based protocol using CellROX Green that can be used for the real-time measurement of general ROS levels of viable suspension mammalian cells. With menadione being the oxidative stress inducer, a cell concentration of 100,000 cells per well, a C...

Upper gastrointestinal endoscopy (GIE) is essential for diagnosing and treating gastrointestinal disorders in children aged 6–12 years, yet it often requires sedation due to the significant discomfort and pain involved. We conducted a PRISMA 2020–compliant systematic review of randomized controlled trials (RCTs) from PubMed, Web of Science, Scopus, and Ovid (inception to March 30, 2024). Inclusion criteria are as follows: RCTs...