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Journal of Fish Diseases · 2023 · Vol. 46 · Issue 11 · Wiley
Yellowfin seabream ( Acanthopagrus latus ) is one of the most commercially important marine fish in China. In this study, a new continuous cell line, named ALS cells, was developed from the spleen tissue of A. latus . The cell line was maintained in Dulbecco's modified Eagle medium/Nutrient Mixture F‐12 Ham (DMEM/F‐12) supplemented with 10% fetal bovine serum (FBS) and successfully cultured up to 50 passages. The cell line was...
Journal of Fish Diseases · 2023 · Vol. 46 · Issue 3 · Wiley
Mandarinfish ranavirus (MRV), also known as a variant of largemouth bass virus (LMBV), is an emerging pathogen in mandarinfish aquaculture. In this study, monoclonal antibodies (mAbs) against MRV were produced and characterized, and 7 mAbs were obtained through Western blotting screening and all 7 mAbs specifically recognized MRV/LMBV but not several piscine iridoviruses as ISKNV, GIV and TFV. By LC MS/MS analysis, the recogni...