DNAzymes have been widely and effectively used for the detection of pathogenic bacteria, which pose a serious public health threat. However, the rapid and cost‐effective detection of such bacteria remains a major challenge. In this study, we successfully selected Vibrio alginolyticus ‐specific DNAzymes. The activity of the candidates was assessed via fluorescence intensity and gel electrophoresis. The DNAzyme DT1 had a detecti...