Photobacterium damselae subsp. piscicida (Pdp) is characterised by high infectivity and mortality and wide distribution, resulting in substantial economic losses to the global aquaculture industry. Therefore, it is critical to develop a simple, rapid and accurate diagnostic method for Pdp detection. Herein, two Pdp‐specific monoclonal antibodies (mAbs 1H7 and 6E6) were obtained, indirect enzyme‐linked immunosorbent assay and i...