The Otocolobus manul , a small felid endemic to the high‐altitude Qinghai‐Tibet Plateau, lacks a high‐quality chromosome‐level genome, hindering study of its evolutionary adaptations. This study aimed to construct such a genome and investigate its genetic basis for high‐altitude adaptation. We generated a de novo chromosome‐scale assembly using an integrated approach of PacBio HiFi long‐reads, Illumina short‐reads, and Hi‐C sc...