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Tick-Borne Pathogens Screening Using a Multiplex Real-Time Polymerase Chain Reaction-Based MethodNARA Subscribed
Purpose This study aims to develop and evaluate a cost-effective, user-friendly multiplex quantitative real-time polymerase chain reaction (qPCR) method for detecting multiple tick-borne pathogens associated with human and veterinary diseases. Methods In silico PCR was performed to design and evaluate primer sequences reported for amplifying Rickettsia spp ., Borrelia spp. , and Ehrlichia spp. Single and multiplex qPCR assays...
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