This study examined how cationic α‑helical antimicrobial peptides such as protamine exchange between the aqueous phase and the transmembrane β‑barrel porin OmpF ( E. coli ) at the single‑molecule level. High‑resolution conductance recordings in planar lipid bilayers revealed clear voltage‑ and concentration‑dependent ion current blockages, consistent with transient peptide interactions within the OmpF lumen. Peptide length inf...

Annexin A5 (anxA5) is a marker for apoptosis, but has also therapeutic potential in cardiovascular diseases, cancer, and, due to apoptotic mimicry, against dangerous viruses, which is limited by the short blood circulation. An 864-amino-acid XTEN polypeptide was fused to anxA5. XTEN864-anxA5 was expressed in Escherichia coli and purified using XTEN as tag. XTEN864-anxA5 was coupled with DTPA and indium-111. After intravenous o...