Since 2020, recurrent mortalities have been reported in grow-out farms in the Ruvu River basin in Tanzania’s Pwani region. This study investigated bacterial pathogens associated with these outbreaks. A cross-sectional survey was conducted between March 2022 and November 2023 in four affected tilapia farms. A total of 60 moribund fish were sampled. Clinical examination revealed skin ulcers, hemorrhages, fin erosion, abdominal d...

This study reports the optimization of a cost‐effective one‐step PCR assay for the detection of White Spot Syndrome Virus (WSSV) in wild shrimp populations, providing an accessible alternative to expensive commercial nested PCR kits in Mozambique. Primer sets targeting VP19, VP24, VP26 and VP28 were evaluated, with the VP28_2 primer set demonstrating the highest sensitivity and no cross‐reactivity with Infectious Hypodermal an...

The pathogenesis of Lactococcus garvieae ( L. garvieae ) was assessed in Nile tilapia ( Oreochromis niloticus ) following administration by two different routes of infection (intraperitoneal versus immersion), using 180 fish divided into three groups. The first group of fish was injected intraperitoneally (IP) with 3 × 10 5 colony‐forming units (cfu) of L. garvieae; the second group was infected by immersion (IMM) into water c...

The genus Edwardsiella is one of the major causes of fish diseases globally. Herein, we examined 37 isolates from ten different fish species from India, South Korea and Taiwan to gain insight into their phenotypic and genotypic properties, of which 30 were characterized as E. tarda with phenotypic homology estimated at 85.71% based on API‐20E biochemical tests. Genotyping using 16S rRNA put all isolates together with E. anguil...

Infectious haematopoietic necrosis virus (IHNV) is the causative agent of infectious haematopoietic necrosis, a disease of salmonid responsible for great economic losses. The disease occurs in most parts of the world where rainbow trout is reared but has not been previously reported in Kenya. In this study, rainbow trout fry and growers from two farms in Nyeri County were screened for IHNV. Whole fry ( n = 4 from each farm) an...

A multilocus sequence analysis ( MLSA ) was carried out to delineate Aeromonas hydrophila from fish in Uganda. Five housekeeping genes including recA , gyrB , metG , gltA and pps; and the 16S rRNA gene were amplified and sequenced from a total of nine A. hydrophila isolates. The obtained sequences were edited, and consensus sequences generated for each gene locus. The housekeeping gene sequences were concatenated and phylogene...