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Haploid embryogenic cultures of maize (Zea mays L.): stability, doubling efficiency, and use in gene editingNARA Subscribed
Embryogenic cultures were initiated from haploid and diploid seedlings of maize inbred LH244 and monitored for ploidy stability by flow cytometry over the course of six mo. Both culture types were transformed with three different constructs using Agrobacterium tumefaciens to compare transformation and gene editing frequencies. Paromomycin resistant cell lines were split prior to plant regeneration, and half of the tissue was t...
We conducted a manipulative nutrient enrichment study to examine the physiological and growth dynamics of the common tropical reef alga Halimeda tun a from shallow and deep coral reef environments on Conch Reef, in the Florida Keys. Paradoxically, H. tuna exhibited higher growth rates at depth (low light and below saturating irradiances) than at shallow sites (high light and at or above saturating irradiances). We hypothesized...
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