Background Quantifying muscle satellite cell proliferation and differentiation is crucial for applications in muscle regeneration, disease modeling, and cultivated meat research. Traditional fluorescence-based assays, while sensitive, are labor-intensive, endpoint-restricted, and disruptive to myotube integrity. Methods In this study, we present a novel high-contrast brightfield (HCBF) imaging technique for high-throughput, la...

Purpose Cultured meat is produced by cultivating animal cells in a bioreactor in a culture medium that provides nutrients and growth factors. Among other animal sera, fetal bovine serum (FBS) has traditionally been the most common used in the culture medium of mammalian cell cultures, i.e., 10% FBS medium that contains 10% FBS and 90% DMEM/F12 (v/v). As the aim of cultured meat is to replace livestock production, animal compon...