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Rapid and user‐friendly diagnostic tests are necessary for early diagnosis and immediate detection of diseases, particularly for on‐site screening of pathogenic microorganisms in aquaculture. In this study, we developed a dual‐sample microfluidic chip integrated with a real‐time fluorogenic loop‐mediated isothermal amplification assay (dual‐sample on‐chip LAMP) to simultaneously detect 10 pathogenic microorganisms, that is Aer...
Summary SR protein‐specific kinases (SRPKs) uniquely with a spacer region are important splicing factors from yeast to human. However, little is known about their biological functions in filamentous fungi. Therefore, we characterized a SRPK called SRK1 in wheat scab fungus Fusarium graminearum . Our data showed that Srk1 is required for vegetative growth, sexual reproduction and plant infection, and plays critical roles in pre...