Many components of the Earth system feature self-reinforcing feedback processes that can potentially scale up a small initial change to a fundamental state change of the underlying system in a sometimes abrupt or irreversible manner beyond a critical threshold. Such tipping points can be found across a wide range of spatial and temporal scales and are expressed in very different observable variables. For example, early-warning...

Apple replant disease (ARD) negatively affects growth and yield of apple plants worldwide. Fungi belonging to the Nectriaceae have often been isolated from roots grown in replant soils and thus are proposed among others as one biotic cause of the disease complex. Microscopic analyses of ARD-affected roots revealed characteristic symptoms associated with fungal infection sites. Here, two extraction methods of such tissue sites...