Radiolabeled RGD peptides targeting expression of α v β 3 integrin have been applied to in vivo imaging of angiogenesis. However, there is a need for more information on the quantitative relationships between RGD peptide uptake and the dynamics of angiogenesis. In this study, we sought to measure the binding of [ 68 Ga]NODAGA-RGDyK to α v β 3 integrin in a human cell-based three-dimensional (3D) in vitro model of angiogenesis,...