We investigated the autumn-to-winter evolution of water-mass structure and nutrient concentrations in Funka Bay, southwestern Hokkaido, Japan, from October to February (2012–2019). Hydrographic and biogeochemical profiles show a recurrent seasonal transition from strongly stratified conditions in October, with low surface nutrients and bottom enrichment, to increasingly homogeneous distributions by mid-winter as vertical mixin...

For decades, leak detection methods utilizing pressure variations (transient test-based technique: TTBT) have been recognized as convenient and accurate for pipeline leak detection. However, their effectiveness in long-distance complex structured field pipelines has not been fully demonstrated, presenting significant challenges for practical implementation. This study, for the first time, tested the effectiveness of inverse tr...

An enteroinvasive Escherichia coli (EIEC) outbreak affecting 154 individuals was identified among attendees at a wedding in Loei Province, Thailand. The median time to symptom onset was 18 h (range: 7–72 h). The epidemiological evidence suggested that larb-neua-dib ( spicy minced raw beef salad) was the probable source of the outbreak. The O8:H19 isolates identified in this study closely resemble the O8:H19 strains from the Un...

Light is one of the most critical stimuli in the majority of living organisms. In the last two decades, blue light (BL) has become a major subject of attention because of developments in light-emitting diodes (LED). The effects of BL on eukaryotic organisms and phototrophic prokaryotes have been well studied, but the knowledge of its effects on non-phototrophic prokaryotes remains unclear. Since BL can penetrate seawater, it i...

Sequencing the entire RNA molecule leads to a better understanding of the transcriptome architecture. SMARTer (Switching Mechanism at 5′-End of RNA Template) is a technology aimed at generating full-length cDNA from low amounts of mRNA for sequencing by short-read sequencers such as those from Illumina. However, short read sequencing such as Illumina technology includes fragmentation that results in bias and information loss....