Zusammenfassung Das Projekt NIKE-MED zielt darauf ab, die Effizienz und Effektivität der Einsatzkräfte bei der Bewältigung von Notfällen insbesondere bei Großschadensereignissen zu erhöhen, um die Sicherheit der betroffenen Personen zu gewährleisten. Komplexe Einsätze unter Tage sind mit einem Massenanfall von Opfern mit sehr spezifischen Verletzungsmustern, die man aus bewaffneten Konflikten kennt (Vergiftungen, Verbrennungen...

Objective To test whether recombinant human diamine oxidase (rhDAO) with a mutated heparin-binding motif (mHBM), which shows an increased alpha-distribution half-life, prevents histamine-induced hemodynamic effects. Material Thirty-eight female guinea pigs were either pretreated with rhDOA_mHBM or buffer. Treatment and methods Guinea pigs received a continuous infusion of histamine. Heart rate (HR), body core temperature and m...

Micro- and nanoplastics (MNPs) are recognized as emerging contaminants, especially in food, with unknown health significance. MNPs passing through the gastrointestinal tract have been brought in context with disruption of the gut microbiome. Several molecular mechanisms have been described to facilitate tissue uptake of MNPs, which then are involved in local inflammatory and immune responses. Furthermore, MNPs can act as poten...

Objective To evaluate the contribution of endogenous diamine oxidase (DAO) in the inactivation of exogenous histamine, to find a mouse strain with increased histamine sensitivity and to test the efficacy of rhDAO in a histamine challenge model. Methods Diamine oxidase knockout (KO) mice were challenged with orally and subcutaneously administered histamine in combination with the β-adrenergic blocker propranolol, with the two h...

Objective To measure diamine oxidase (DAO) activity with high sensitivity in complex matrices like plasma or tissue extracts radioactive putrescine or horseradish peroxidase (HRP)/hydrogen peroxide (H 2 O 2 ) coupling must be used. The use of radioactive material should be avoided and HRP/H 2 O 2 coupling is compromised by antioxidants. Methods and results Condensation of ortho-aminobenzaldehyde (oABA) with delta-1-pyrroline a...