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Unraveling geographic distribution patterns of planktonic protists is a central goal in marine microbial ecology. Using a novel combination of recently developed phylogenetic and network analyses on a V4 18S rDNA metabarcoding dataset, we here analyzed the genetic diversity of marine planktonic ciliate communities in Chinese and European coastal waters. Thereby, our approach provided an unprecedented perspective on geographic...
Aquatic food webs in deep temperate lakes: Key species establish through their autecological versatilityNARA Subscribed
Microbial planktonic communities are the basis of food webs in aquatic ecosystems since they contribute substantially to primary production and nutrient recycling. Network analyses of DNA metabarcoding data sets emerged as a powerful tool to untangle the complex ecological relationships among the key players in food webs. In this study, we evaluated co‐occurrence networks constructed from time‐series metabarcoding data sets (1...
Is repeated pulmonary metastasectomy justified?NARA Subscribed
Recurrence after pulmonary metastasectomy (PM) is frequent, but it is unclear to whom repeated pulmonary metastasectomy (RPM) offers highest benefits. Retrospective analysis of oncological and post-operative outcomes of consecutive patients who underwent PM from 2003 to 2018. Overall survival (OS) and disease-free interval (DFI) were calculated. Cox regression was used to identify variables influencing OS and DFI. In total, 26...
Improving eDNA‐based protist diversity assessments using networks of amplicon sequence variantsNARA Subscribed
Summary Effective and precise grouping of highly similar sequences remains a major bottleneck in the evaluation of high‐throughput sequencing datasets. Amplicon sequence variants (ASVs) offer a promising alternative that may supersede the widely used operational taxonomic units (OTUs) in environmental sequencing studies. We compared the performance of a recently developed pipeline based on the algorithm DADA2 for obtaining ASV...
Transition boundaries for protistan species turnover in hypersaline waters of different biogeographic regionsNARA Subscribed
Summary The identification of environmental barriers which govern species distribution is a fundamental concern in ecology. Even though salt was previously identified as a major transition boundary for micro‐ and macroorganisms alike, the salinities causing species turnover in protistan communities are unknown. We investigated 4.5 million high‐quality protistan metabarcodes (V4 region of the SSU rDNA) obtained from 24 shallow...
Marine protist diversity in E uropean coastal waters and sediments as revealed by high‐throughput sequencingNARA Subscribed
Summary Although protists are critical components of marine ecosystems, they are still poorly characterized. Here we analysed the taxonomic diversity of planktonic and benthic protist communities collected in six distant E uropean coastal sites. Environmental deoxyribonucleic acid ( DNA ) and ribonucleic acid ( RNA ) from three size fractions (pico‐, nano‐ and micro/mesoplankton), as well as from dissolved DNA and surface sedi...
Contributory presentations/postersNARA Subscribed
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