Background Mycobacterium bovis BCG is widely used as a vaccine vector due to its safety, genetic stability, and immunomodulatory properties. However, current expression systems for BCG transformation rely on antibiotic resistance genes, raising biosafety concerns. Therefore, alternative strategies—such as the use of auxotrophic strains—are highly desirable. Our group previously employed BioBrick™ technology to construct the pU...

Postbiotics, including inactivated microorganisms and their bioactive components, have emerged as innovative candidates for microbiota-targeted therapies. Among them, Lactobacillus reuteri -derived postbiotics represent a promising and underexplored strategy for modulating host physiology without the risks associated with live bacteria. Selenium enrichment may further enhance these benefits by strengthening antioxidant defense...

We developed a species‐by‐species approach for selecting protected areas for conservation of native freshwater fishes in semiarid regions, with catchment as the fundamental landscape unit for conservation efforts. Input data were composed of occurrences of freshwater fishes and landscape variables, and general quantification of occurrence, abundance, and endemicity of each fish species, to derive an index of conservation value...