Cannabis Use Disorder (CUD) affects ~ 50 million individuals worldwide and is associated with alterations in brain reward pathways. Mindfulness-based interventions (MBIs) show promise in reducing substance use and aberrant brain function in substance use disorders (SUD), but the effects on CUD or brain reward function have not been investigated. To examine whether a 2-week MBI vs. active control (i.e., closely matched relaxati...

A reliable and simple Agrobacterium -mediated transformation system for the unicellular green algae model organism Chlamydomonas reinhardtii has been developed. The protocol has been successfully employed with both neomycin phosphotransferase II ( nptII ) and the phleomycin resistance ( bleI ) genes coupled with the selective agents paromomycin and zeocin, respectively. A set of binary vectors were assembled that carry the sel...