This study presents the first reproducible vitrification-based cryopreservation protocol for shoot tips of sycamore maple ( Acer pseudoplatanus L.), a tree of high ecological and economic significance in Central Europe. By safeguarding genetically verified material, including rare wood traits, this work supports the integration of the species into long-term ex situ conservation programmes. We investigated the influence of expl...

Sycamore maple ( Acer pseudoplatanus L.) is an ecologically and economically important hardwood in Central Europe. For high-value genotypes, like wavy grain maple, in vitro propagation offers a promising tool for targeted multiplication and conservation. This study contributed to an optimised micropropagation protocol by testing disinfection protocols, silver nitrate supplementation, cytokinin type, and light conditions. Winte...

Introduction Apple replant disease (ARD) causes reduced growth and fruit yield and affects orchards and tree nurseries worldwide. A number of pathogens have been consistently identified as causal agents of ARD; however factors affecting disease-severity are not fully understood. Aims We examined five soils from German tree nurseries and apple orchards featuring different soil characteristics and replant histories. We aimed to...

Key Message Multiple QTLs reveal the polygenic nature of R. rhizogenes -mediated transformation and hairy root formation in roses, with five key regions explaining 12.0–26.9% of trait variability and transformation-related candidate genes identified. Abstract Understanding genetic mechanisms of plant transformation remains crucial for biotechnology. This is particularly relevant for roses and other woody ornamentals that exhib...

This study explored the establishment and optimization of Ri (root inducing) technology for apple breeding, using the bacterium Rhizobium rhizogenes to obtain Ri lines with compact shoots and stronger root systems. The transformation and shoot regeneration for Malus domestica cultivars was studied in detail. Various R. rhizogenes strains, scion and rootstock genotypes, explant types, wounding methods and explant orientations w...

Because of their shallow root system, drought stress is a major problem in potato cultivation. Due to climate change more severe drought periods are expected to occur in the vegetative growth phase of potato growth. Therefore, there is a great need for drought tolerant potato genotypes. Potato responds to drought stress in the field in various ways, including osmoregulation. Osmotic stress can be induced in vitro by adding an...

Hyperhydricity (HH) is one of the most important physiological disorders that negatively affects various plant tissue culture techniques. The objective of this study was to characterize optical features to allow an automated detection of HH. For this purpose, HH was induced in two plant species, apple and Arabidopsis thaliana , and the severity was quantified based on visual scoring and determination of apoplastic liquid volum...

Main conclusion Nitrogen deficient and drought-tolerant or sensitive potatoes differ in proteomic responses under combined (NWD) and individual stresses. The sensitive genotype ‘Kiebitz’ exhibits a higher abundance of proteases under NWD. Abstract Abiotic stresses such as N deficiency and drought affect the yield of Solanum tuberosum L. tremendously. Therefore, it is of importance to improve potato genotypes in terms of stress...

In a diversity panel of 96 rose genotypes, variation in the capacity to form calluses on leaf explants in vitro was investigated, and a genome-wide association study (GWAS) was performed to identify genetic factors associated with callus formation. Calluses were induced from wounded in vitro leaflets on two media differing in their plant growth regulator composition. Significant differences between genotypes were observed in c...