Ageratum enation virus (AEV) ( Begomovirus agerati) , belonging to the genus Begomovirus , is associated with DNA components known as alphasatellite and betasatellites. The alphasatellite encodes the replication-associated protein (alpha-Rep), which is essential for self-replication but relies on helper viruses for transmission between plants. In this study, we propose that the alpha-Rep promoter exhibits bidirectional promote...