Background Betacellulin (BTC), an EGF-family growth factor expressed in granulosa cells, is implicated in ovarian regulation; however, its interactions with gonadotropins in humans remain unclear. Objective To determine how BTC alone or combined with follicle-stimulating hormone (FSH) or luteinizing hormone (LH) affects human granulosa cell viability, proliferation, apoptosis, and progesterone secretion. Methods Human granulos...

Background Circular RNAs (circRNAs) are stable epigenetic regulators of various biological processes, yet the functional role of ciR-02852 remains unknown. This study aimed to characterize the influence of ciR-02852 on the fundamental functions of porcine ovarian granulosa cells. Methods Cells were transfected with either a ciR-02852-overexpressing vector or an shRNA ciR-02852-knockdown vector. We assessed cell viability, prol...

Keymessage Standardized microhaplotype databases for eight diverse crops enable multiallelic analyses, comparative genetics, and breeding decisions. Abstract Microhaplotypes are short genomic segments that contain multiple tightly linked variants, providing multi-allelic data that can enhance genetic resolution compared to traditional biallelic single nucleotide polymorphism (SNP) markers. Here, we present the creation and uti...