This study evaluated the possible use of the fish SSN‐1 cell line to investigate the development of Macrobrachium rosenbergii nodavirus ( Mr NV). Cells were incubated with viral particles and cytopathic effects were observed. De novo synthesis of viral capsid proteins was shown by immuno‐fluorescence labelling and a sandwich ELISA test. Viral genomic replication was demonstrated by RT‐PCR using primers specific to RNA‐1 as wel...