V. Lucchini results 10
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Molecular analysis of metallo-beta-lactamase-producing Pseudomonas aeruginosa in Switzerland 2022–2023NARA Subscribed
Objectives The occurrence of metallo-beta-lactamase-producing Pseudomonas aeruginosa (MBL-PA) isolates is increasing globally, including in Switzerland. The aim of this study was to characterise, phenotypically and genotypically, the MBL-PA isolates submitted to the Swiss National Reference Center for Emerging Antibiotic Resistance (NARA) reference laboratory over a 12-month period from July 2022 to July 2023. Methods Thirty-n...
Multinational evaluation of the BioFire® FilmArray® Pneumonia plus Panel as compared to standard of care testingNARA Subscribed
This study compared standard of care testing (SOC) to BioFire® FilmArray® Pneumonia plus Panel (PN plus ). PN plus detects 15 bacteria with semiquantitative log bin values, 7 antibiotic resistance markers, three atypical bacteria (AB), and eight viral classes directly from bronchoalveolar lavage-like specimens (BLS) and sputum-like specimens (SLS). Fifty-two laboratories from 13 European countries and Israel tested 1234 BLS an...
Occasional crossbreeding between free‐ranging domestic dogs and wild wolves ( Canis lupus ) has been detected in some European countries by mitochondrial DNA sequencing and genotyping unlinked microsatellite loci. Maternal and unlinked genomic markers, however, might underestimate the extent of introgressive hybridization, and their impacts on the preservation of wild wolf gene pools. In this study, we genotyped 220 presumed I...
Evidence of genetic distinction and long‐term population decline in wolves ( Canis lupus ) in the Italian ApenninesNARA Subscribed
Historical information suggests the occurrence of an extensive human‐caused contraction in the distribution range of wolves ( Canis lupus ) during the last few centuries in Europe. Wolves disappeared from the Alps in the 1920s, and thereafter continued to decline in peninsular Italy until the 1970s, when approximately 100 individuals survived, isolated in the central Apennines. In this study we performed a coalescent analysis...
Phylogeography of the rock partridge ( Alectoris graeca )NARA Subscribed
We used mitochondrial DNA control‐region and microsatellite data to infer the evolutionary history and past demographic changes in 332 rock partridges ( Alectoris graeca ) sampled from throughout the species’ distribution range, with the exception of the central Balkans region. Maternal and biparental DNA markers indicated concordantly that rock partridge populations are structured geographically (mtDNA φ ST = 0.86, microsatel...
Noninvasive molecular tracking of colonizing wolf ( Canis lupus ) packs in the western Italian AlpsNARA Subscribed
We used noninvasive methods to obtain genetic and demographic data on the wolf packs ( Canis lupus ), which are now recolonizing the Alps, a century after their eradication. DNA samples, extracted from presumed wolf scats collected in the western Italian Alps (Piemonte), were genotyped to determine species and sex by sequencing parts of the mitochondrial DNA (mtDNA) control‐region and ZFX/ZFY genes. Individual genotypes were i...
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