Introduction Monitoring of estuarine fish biodiversity is often constrained by the inherent limitations of traditional survey methods and the complex, dynamic environmental conditions of estuarine habitats. Environmental DNA (eDNA) metabarcoding has emerged as a robust molecular tool for aquatic biodiversity assessment. Nevertheless, its complementary potential to conventional bottom trawling remains understudied in estuarine...

This study investigates the spatiotemporal density aggregation and pattern distribution of vessel traffic amidst bustling maritime logistics scenarios. Firstly, a relatively new spatiotemporal segmentation and reconstruction method is proposed for ship AIS trajectories to address trajectory disruptions caused by berthing, anchorage, and other factors. Subsequently, a trajectory filtering algorithm utilizing time window panning...