Five cell lines (LJHK, LJS, LJL, LJH‐1 and LJH‐2) were established from the head kidney, spleen, liver and heart of sea perch Lateolabrax japonicus . The cell lines LJHK, LJS, LJL, LJH‐1 and LJH‐2 were subcultured 46, 32, 32, 36 and 34 times in minimum essential medium (MEM) supplemented with foetal bovine serum (FBS), sea perch serum and 10 ng ml −1 basic fibroblast growth factor (bFGF). Morphology of primary cultures and sub...

A pluripotent cell line, sea bream embryonic stem‐like cells (SBES1), was developed from blastula‐stage embryos of the cultured red sea bream, Chrysophrys major . The SBES1 cells were cultivated in Dulbecco's modified eagles medium (DMEM) medium supplemented with foetal bovine serum, marine fish serum, fish embryo extract, selenium, basic fibroblast growth factor and leukemia inhibitory factor. They were small and round or pol...