Using a three-factor orthogonal design, we investigated the transcriptomic and histological responses of triploid rainbow trout (Oncorhynchus mykiss) gills across three salinities (10, 20, and 30 ppt) over 60 days, with temperature and body size as background conditions. Results showed that 20 ppt was associated with extensive transcriptional reprogramming, with 3333 differentially expressed transcripts (DETs), whereas 30 ppt...

(1) Background: Vaccination is crucial for Ctenopharyngodon idella, yet the impact of delivery methods on welfare and immunity requires investigation. (2) Methods: This study compared manual (HI-V) and automated (AI-V) injections of inactivated Aeromonas hydrophila vaccine. (3) Results: Both methods induced protective immunity, with AI-V achieving a slightly higher Relative Percent Survival (41.67%) than HI-V (37.84%). AI-V pr...