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Environmental Microbiology · 2025 · Vol. 27 · Issue 12 · Wiley
In cold seeps, anaerobic methanotrophic archaea (ANME) and sulphate‐reducing bacteria (SRB) oxidise methane to inorganic carbon (IC) coupled to sulphate reduction. While catabolic pathways are well resolved, carbon flow into biomass as well as the functional roles of lipid biomarkers remain unclear. We conducted lipid stable isotope probing (lipid‐SIP) experiments with Astoria Canyon sediments dominated by ANME‐2/SRB consortia...