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Journal of Fish Diseases · 2026 · Vol. 49 · Issue 10 · Wiley
The infectious hypodermal and haematopoietic necrosis virus (IHHNV) represents a significant viral threat to global shrimp aquaculture, leading to considerable economic losses. In this study, we have developed a one‐step, one‐pot isothermal assay for the detection of IHHNV, employing recombinase‐aided amplification in conjunction with clustered regularly interspaced short palindromic repeats‐Cas12a (RAA‐CRISPR/Cas12a). The ass...