Viral hemorrhagic septicemia virus (VHSV) is a highly pathogenic rhabdovirus that primarily spreads through horizontal transmission. Characterizing viral shedding dynamics is therefore critical for understanding its epidemiology and improving disease control strategies in aquaculture. In this study, the median lethal dose (LD₅₀) of VHSV in olive flounder ( Paralichthys olivaceus ) was determined to be 10 3.7 TCID 50 /100 μL. W...

Viral hemorrhagic septicemia virus (VHSV) is a major pathogen in olive flounder ( Paralichthys olivaceus ) aquaculture, leading to high mortality rates and significant economic losses. Recurrent outbreaks underscore the need for a deeper understanding of the early‐stage adhesion and infection mechanisms of VHSV. To address this gap, this study investigated the adsorption and replication dynamics of VHSV in various olive flound...