Only a few human ovarian endometrioid carcinoma cell lines are currently available, partly due to the difficulty of establishing cell lines from low-grade cancers. Here, using a cell immortalization strategy consisting of i) inactivation of the p16 INK4a -pRb pathway by constitutive expression of mutant cyclin-dependent kinase 4 (R24C) (CDK4 R24C ) and cyclin D1, and ii) acquisition of telomerase reverse transcriptase (TERT) a...

Background Understanding how healthy articular cartilage responds to mechanical loading is critical. Moderate mechanical loading has positive effects on the cartilage, such as maintaining cartilage homeostasis. The degree of mechanical loading is determined by a combination of intensity, frequency, and duration; however, the best combination of these parameters for knee cartilage remains unclear. This study aimed to determine...

A human ovarian clear cell carcinoma cell line was established from a 46-year-old Japanese woman. That line, designated MTC-22, has proliferated continuously for over 6 months in conventional RPMI 1640 medium supplemented with 10% foetal bovine serum and has been passaged over 50 times. MTC-22 doubling-time is ~ 18 h, which is much shorter than most ovarian clear cell carcinoma lines reported to date. Morphologically, MTC-22 c...

We examined long‐term charcoal records spanning the glacial–interglacial cycles that are evident in two cores collected from Lake Biwa in central Japan. We found that the records of the two cores have a similar long‐term variation pattern of charcoal concentrations and abundant large charcoal fragments in postglacial sediments, which indicates that frequent fires occurred near the shores of Lake Biwa during the postglacial per...

Vitamin A levels in fattening Japanese Black cattle affect meat quality; therefore, it is important to monitor serum retinol concentrations. To simplify and accelerate the evaluation of serum retinol concentrations in cattle, we developed a new predictive method using excitation-emission matrix (EEM) fluorescence spectrophotometry. For analytical comparison, the concentration of serum retinol was also measured using the conven...