Simulated manipulation experiments, such as nitrogen addition to mimic atmospheric nitrogen deposition, are widely used in global change research. However, experimental manipulations may differ from real‐world environmental change in their intensity, duration, and co‐occurrence, leaving long‐term changes in soil microbial communities and soil health insufficiently understood. To address this gap, we resampled soils from 38 for...

Photobacterium damselae subsp. damselae (PDD), a marine fish pathogen, has an unclear contribution of tonB genes to iron acquisition and pathogenicity. ΔtonB1-PDD and ΔtonB2-PDD mutants as well as their complemented strains were generated via homologous recombination from the virulent strain PDD1605. Growth was evaluated by monitoring OD600 with viable-count calibration, intracellular iron was measured using a colorimetric ass...

Understanding long‐term phytoplankton community dynamics is essential for assessing ecological resilience and informing lake management under global change. However, centennial‐scale patterns of phytoplankton dynamics remain poorly resolved due to the limited taxonomic resolution of traditional fossil‐based paleoecological approaches. In this study, a combination of sedimentary DNA (sedDNA) and subfossil diatom analysis was us...