Summary Lysine 2‐hydroxyisobutyrylation (K hib ) is a recently identified post‐translational modification (PTM) that regulates numerous cellular metabolic processes. In pathogenic microorganism, although glycolysis and fungal virulence are regulated by K hib , its potential roles in fungi remain to be elusive. Our preliminary results showed that levels of K hib fluctuate over time in Aspergillus flavus , which frequently conta...