Z. Sha results 14
· Newest (Page 1/1, per page 25)
Author: Z. Sha ×Clear All Filters
Search Results
Streptococcus pneumoniae is a highly invasive bacterial pathogen that can cause a range of illnesses. Pneumococcal capsular polysaccharides (CPS) are the main virulence factors that causes invasive pneumococcal disease (IPD). Pneumococcal CPS serotype 7F along with a few other serotypes is more invasive and likely to cause IPD. Therefore, 7F is a target for pneumococcal vaccine development, and is included in the two recently...
Establishment and characterization of an ovarian cell line from half‐smooth tongue sole Cynoglossus semilaevisNARA Subscribed
A new ovarian cell line, CSO , was established from half‐smooth tongue sole Cynoglossus semilaevis . Primary culture of CSO cells was initiated from digestion of ovarian tissues pieces by trypsin solution and cultured at 24° C in Dulbecco's modified Eagle's medium– F12 medium ( DMEM – F12 , 1:1) (pH 7·0), supplemented with 20% foetal bovine serum, basic fibroblast growth factor ( bFGF ), epidermal growth factor ( EGF ), insuli...
Three normalized cDNA libraries were constructed, two of which were constructed from reproductive tissues ovary and testis, and the other one from pooled immune tissues including head kidney, intestine, liver and spleen. A total of 10 542 clones were sequenced generating 10 128 expressed sequence tags (ESTs). Cluster analysis indicated a total of 5808 unique sequences including 1712 contigs and 4096 singletons. A total of 4249...
Development and characterization of cell lines from heart, liver, spleen and head kidney of sea perch Lateolabrax japonicusNARA Subscribed
Five cell lines (LJHK, LJS, LJL, LJH‐1 and LJH‐2) were established from the head kidney, spleen, liver and heart of sea perch Lateolabrax japonicus . The cell lines LJHK, LJS, LJL, LJH‐1 and LJH‐2 were subcultured 46, 32, 32, 36 and 34 times in minimum essential medium (MEM) supplemented with foetal bovine serum (FBS), sea perch serum and 10 ng ml −1 basic fibroblast growth factor (bFGF). Morphology of primary cultures and sub...
A pluripotent cell line, sea bream embryonic stem‐like cells (SBES1), was developed from blastula‐stage embryos of the cultured red sea bream, Chrysophrys major . The SBES1 cells were cultivated in Dulbecco's modified eagles medium (DMEM) medium supplemented with foetal bovine serum, marine fish serum, fish embryo extract, selenium, basic fibroblast growth factor and leukemia inhibitory factor. They were small and round or pol...
Previous1Next