Plasmid‐mediated conjugation is an important pathway for the spread of antibiotic resistance genes (ARGs), posing a significant risk to global public health. It has been reported that the conjugative transfer of ARGs could be enhanced by oxidative stress. Whether endogenous glutathione (GSH), a major non‐protein thiol compound involved in cellular redox homeostasis, influences conjugative transfer is unknown. In this study, we...