microRNA (miRNA) is a type of endogenous short-chain non-coding RNA with regulatory function found in eukaryotes, which is involved in the regulation of a variety of cellular and biological processes. However, the research on the development of cashmere goat secondary hair follicles is still relatively scarce. In this study, small RNA libraries and mRNA libraries of 45 days, 55 days, 65 days, and 75 days of fetal skin of cashm...

Invasive Salmonella infections result in a significant burden of disease including morbidity, mortality, and financial cost in many countries. Besides typhoid fever, the clinical impact of non-typhoid Salmonella infections is increasingly recognized with the improvement of laboratory detection capacity and techniques. A retrospective multicenter study was conducted to analyze the clinical profiles and antimicrobial resistance...

The effects of various levels of alkalinity stress (0, 18.25, 35.41, 52.53 and 69.74 mmol/L) in Chinese mitten crab (Eriocheir sinensis) were investigated by means of measuring hepatopancreas antioxidant system and serum nonspecific immune system-related indices at 0, 12, 24, 48 and 96 h, hepatopancreas tissue structure at 96 h. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and aspartate amino...

There has been little systematic analysis of the influence of the three‐step staircase topography on the characteristics of precipitation along latitude 30°N in East Asia. After the distribution of atmospheric climate states over East Asia was analysed, in this study, the climatological characteristics of summer convective and stratiform precipitation on horizontal distribution, vertical structure, and diurnal variation in Eas...

The non-hydrostatic global variable resolution model (MPAS-atmosphere) is used to conduct the simulations for the South Asian Summer monsoon season (June, July, and August) in 2015 with a refinement over the Tibetan Plateau (TP) at the convection-permitting scale (4 km). Two experiments with different topographical datasets, complex (4-km) and smooth (60-km) topography, are designed to investigate the impacts of topographical...

In order to scientifically and reasonably evaluate the safety risk of ship navigation, to and better solve the problems of the poor sensitivity of static evaluation and insufficient ability to grasp the overall dynamic situation, in this paper, representative safety evaluation indexes for ship navigation are screened and the initial weight of each index is confirmed in combination with the improved analytic hierarchy process (...

Genetic manipulation of Haematococcus pluvialis is difficult because of the lack of a stable and convenient transformation system. The pH124-EGFP-Ble vector containing ble as a selective gene and EGFP as a reporter gene was constructed and employed for effective transformation. H. pluvialis protoplasts were obtained by treating with cellulase and macerozeme. Then polyethylene glycol-mediated transformation was established by i...