HDV has a high genetic diversity among isolates and a high capacity to accumulate mutations over time. The mechanisms involved in the clinical progression of HDV are not fully elucidated. Few studies relate mutations to clinical implications, which makes this variable extremely relevant for research into HDV-3, a genotype associated with worse clinical progression. The aim of this study was to analyze mutations in complete gen...

This study reports the first re-isolation of a wild-type Salehabad virus (SALV) since 1959, from a pool of male sand flies collected in Türkiye in August 2023. The isolate and the 1959 prototype strain shared 86–92% nucleotide identity across the four coding sequences but 95–99% amino acid identity; for the L protein, 12% nucleotide divergence corresponded to 1% amino acid divergence. Because male sand flies do not blood-feed,...

Viruses from wild plants may emerge as new economically important agricultural pathogens through spillover events. Blainvillea yellow spot virus ( Begomovirus blainvilleae , BlYSV) is a bipartite geminivirus which is ubiquitous in the non-cultivated plant Blainvillea rhomboidea . To better understand the ecological and evolutionary aspects of this virus, we sampled B. rhomboidea in three Brazilian states between 2020 and 2023....

Accurate detection of high-risk human papillomavirus (HPV) is central to cervical cancer screening. Current diagnostic strategies increasingly prioritize broad-spectrum assays to maximize genotype coverage. However, theoretical genotype breadth does not necessarily translate into effective sample-level detection when clinically dominant targets are present at low copy number. Here, we evaluated this analytical trade-off using...

For the first time sequence data of a fabavirus was identified in dahlia from the UK, which was similar to virus sequence reported from dahlia in China. Initial sequences were determined by high-throughput sequencing (HTS) using the Illumina MiSeq platform, and were verified by Sanger sequencing and 5’ and 3’ rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR). The genome comprised two RNAs that were 5,851...