Antimicrobial resistance (AMR) is a major global health concern, and its monitoring in wildlife is essential from a One Health perspective. Here, we obtained bacterial isolates and assessed the AMR profiles and extended-spectrum β-lactamase (ESBL) production of Escherichia coli isolated from fecal samples of wild felids in a protected Atlantic Forest area in southeastern Brazil. We collected 22 fecal samples and identified dep...

This study evaluated the anesthetic combination of dexmedetomidine and ketamine in free-ranging Phrynops geoffroanus , assessing cardiopulmonary and sedative variables, as well as pharmacological reversal with atipamezole. Twenty-five adult individuals were allocated into two groups: HG (dexmedetomidine 0.1 mg/kg + ketamine 10 mg/kg; n = 13) and LG (dexmedetomidine 0.05 mg/kg + ketamine 10 mg/kg; n = 12). Heart rate, respirato...

Flies are important vectors of antimicrobial resistance (AMR) in food production, but their role in meat processing environments remains poorly characterized. This study isolated Enterobacteriaceae and Staphylococcaceae (including Mammaliicoccus sciuri ) from flies collected in bovine and swine slaughterhouses and meat processing industries. Phenotypic AMR was assessed, and the presence of selected antimicrobial resistance and...

The expansion of invasive European wild boar ( Sus scrofa scrofa ) populations in Brazil and the increasing commercialization of this species raise concerns about their role as reservoirs of zoonotic and antimicrobial-resistant bacteria. This study aimed to determine the prevalence, antimicrobial resistance profiles, and detection of virulence and resistance genes in Escherichia coli and Salmonella spp. isolated from captive w...

Plant-mediated green synthesis has emerged as a sustainable and cost-effective strategy for the production of gold nanoparticles (AuNPs). In this study, gold nanoparticles were synthesized using an aqueous extract of Virola oleifera resin (AuNPs-Vo), and their synthesis parameters were optimized through factorial experimental design, followed by physicochemical characterization and cytotoxicity assessment. Factorial experiment...

Alphaviruses, including Mayaro virus (MAYV) and Chikungunya virus (CHIKV), pose a growing public health threat, with increasing reports worldwide and no licensed antiviral treatments available. This study evaluated the antiviral activity of pyridine-3-carbohydrazide (P-3-C) against MAYV (strains BeAr and TRVL) and CHIKV (strain La Reunion, LR). Vero E6 or U-2OS cells were infected and treated with P-3-C for 24 h, and cell viab...

Burkholderia cenocepacia is an important pathogen in cystic fibrosis (CF) patients, responsible for chronic lung infections and, in severe cases, cepacia syndrome (CS), characterized by necrotizing pneumonia, bacteremia, and high mortality. Epidemic lineages, such as B. cenocepacia J2315 (ET12) and ST32, are highly transmissible and frequently associated with CS. In this study, five B. cenocepacia isolates recovered from sputu...

This study aimed to evaluate the effect of topical application of a mouth gel formulation containing iron tetracarboxyphthalocyanine (FeTcPc) during non-surgical periodontal treatment. Forty-seven individuals with periodontitis underwent a standard periodontal treatment (scaling and root planing), followed by adjuvant therapy according to the = groups: Vehicle Group ( n = 15), receiving local application of the FeTcPc gel vehi...