This study investigated the diversity of thermophilic Campylobacter species isolated from three New Zealand freshwater catchments affected by pastoral and urban activities. Utilising matrix‐assisted laser desorption ionisation‐time of flight and whole genome sequence analysis, the study identified Campylobacter jejuni ( n = 46, 46.0%), C . coli ( n = 39, 39%), C . lari ( n = 4, 4.0%), and two novel Campylobacter species lineag...