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Enumeration of organisms occurring in the intestinal tract and yolk sac of day-old chicks from commercial hatcheriesNARA Subscribed
This study evaluated the naturally occurring microorganisms within the gastrointestinal tract (GIT) and yolk sacs of day-old commercial broiler chicks. Across 6 independent trials, day-old chicks ( n = 10 per trial) were obtained from various commercial hatcheries, humanely euthanized, and necropsied. The entire intestine (duodenum to colon) and the yolk sac were isolated, homogenized, serially diluted, and plated onto selecti...
Improving inferences from coyote (Canis latrans) surveys across Illinois using integrated state-space modelsNARA Subscribed
Anthropogenic influences on landscapes changed wildlife communities immensely over the last century. One notable example of human-derived change is the expansion of coyote ( Canis latrans ) populations following the extirpation of apex carnivores and the inception of industrial agriculture in North America. In Illinois, USA, the Department of Natural Resources estimates relative abundance of coyotes using a variety of survey t...
Use of translational fusions to express functional Klebsiella oxytoca dinitrogenase reductase in plant mitochondriaNARA Subscribed
Engineering crop plants with the biological nitrogen fixation pathway is a longstanding goal of modern agriculture. Dinitrogenase reductase (NifH) is a critical component of the biological nitrogen fixation pathway, with multiple roles in metal cofactor assembly and catalysis. This enzyme must be folded correctly as a soluble homodimer and loaded with the [4Fe-4S] metallocluster for function. Previous studies have found that K...
Along the Benthic to Holoplanktonic Continuum: Seafloor Affinity Increases Distance‐Decay Rates Among Abyssal CommunitiesNARA Subscribed
Aim Marine species ranges are often large, contributing to relatively high community similarity over space, with decreased ranges expected among taxa that have lower dispersal and/or higher substrate requirements. We tested for a weakening of biogeographic structuring among dispersing abyssal taxa along the benthic to holoplanktonic habitat continuum by comparing geographic ranges and rates of community dissimilarity across th...
Efficacy of an Inactivated Bacterin Vaccine Against Emerging Streptococcus dysgalactiae in Channel Catfish ( Ictalurus punctatus )NARA Subscribed
Lancefield Group C Streptococcus dysgalactiae recently emerged as a catfish pathogen in the US. Reports from hatcheries suggest S. dysgalactiae causes substantial broodfish loss. We aimed to develop a challenge model and evaluate vaccine efficacy against S. dysgalactiae in juvenile channel catfish ( Ictalurus punctatus ). Three S. dysgalactiae (14–1, 14–24 and 14–25) isolates were tested for virulence. Intracoelomic (IC) injec...
Bottom‐tow gear fisheries (trawls and dredges) produce ~24 million mt of harvest annually, representing a globally important animal‐sourced food system. While many are currently sustainably managed, growing concern over the potential for ecosystem impacts from bottom‐tow gears has increased pressure to improve these fisheries. We assembled an international working group of > 30 contributors, including fishing industry particip...
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First direct evidence of spawning by Japanese sardine Sardinops melanosticta (Temminck and Schlegel) in the eastern Pacific OceanNARA Subscribed
In 2022 the presence of the Japanese sardine, Sardinops melanosticta (Temminck and Schlegel), was detected in the eastern Pacific Ocean, far outside its known western North Pacific range. The species was collected along with the Pacific sardine, Sardinops sagax (Jenyns), which is native to the eastern Pacific Ocean. Here, we report the first direct evidence for spawning by S. melanosticta in the eastern Pacific Ocean.
Mescaline Alters Cerebellar Function, Global Connectivity, and Frequency-Selective Acoustic Gating: A BOLD fMRI Study in Awake RatsNARA Subscribed
Mescaline, a 5-HT2A agonist psychedelic used ceremonially for millennia, lacks neuroimaging characterization due to its Schedule 1 status. Using pharmacological and resting-state fMRI in awake rats, we report mescaline's first comprehensive neurobiological profile. Acutely, mescaline produced cerebellar-selective BOLD suppression, suggesting functional disconnection from forebrain structures. Paradoxically, resting-state analy...
Deep-sea whale falls serve as biodiversity hotspots and pass through distinct successional stages, with the duration of the sulphophilic stage remaining controversial. We conducted a 15-year time-series study of a blue or fin whale skeleton on the edge of the Oxygen Minimum Zone at 1288 m depth on the Clayoquot Slope, Cascadia Continental Margin, using ROV surveys and high-resolution 3D photogrammetry to assess long-term ecosy...
We provide best practices for estimating the dissipation rate of turbulent kinetic energy, ε, from velocity measurements in an oceanographic context. These recommendations were developed as part of the Scientific Committee on Oceanographic Research (SCOR) Working Group #160 “Analyzing ocean turbulence observations to quantify mixing”. The recommendations here focus on velocity measurements that enable fitting the inertial subr...