Rhipicephalus linnaei is a tick widely associated with domestic dogs and is of recognized veterinary and public health importance. However, the recent taxonomic delimitation of this species, previously included within the Rhipicephalus sanguineus sensu lato complex, has made the production of species-specific biological data necessary. Therefore, this study aimed to characterize the life cycle parameters and reproductive perfo...

The salivary glands of hematophagous arthropods possess a remarkable molecular diversity, reflecting evolutionary adaptations to blood feeding lifestyle and vector-host interactions. In this study, we characterized the salivary-gland transcriptome of adult female Simulium pertinax , a medically important black fly species widely distributed in South America. Through RNA sequencing and bioinformatics analyses, we identified 15,...

Anesthetics are widely employed in aquaculture and in research environments utilizing fish as experimental models, with the aim of mitigating stress and hypermotility during handling procedures. Among the most commonly used anesthetic agents, eugenol stands out for its suitability across multiple species, enabling safer handling and transportation while minimizing physiological impact on the animals. For the experimental proce...

The d emographic shift toward an older population is accelerating the prevalence of age-related diseases. Precision geromedicine represents a paradigm shift in targeting the biological processes of ageing to optimise health and healthspan, which likely requires multimodal tailored interventions. PROMETHEUS, as part of the XPRIZE healthspan semi-finalist competition, is an 8-week feasibility and exploratory study including 20 m...

In late 2024, a mortality event affecting wild dusky grouper ( Epinephelus marginatus ) was reported across multiple islands of the Azores archipelago, Portugal. Following the previous report of the initial detection of nervous necrosis virus (NNV) infection, biological material was submitted to the National Reference Laboratory, where the outbreak was confirmed and the virus was comprehensively characterized through molecular...

Among the many factors that limit sugarcane crop yield, the precise supply of nutrients and water under drip irrigation systems can be the shortcut to higher yields in the field. This research aimed to evaluate the nutritional efficiency of fertigated sugarcane varieties under deficit irrigation strategies. The experiment was conducted in a rain‐out shelter with the soil type Oxisol Typic Ustox. The experimental design adopted...

The search for safe and effective anaesthetic agents for aquaculture is essential to improve fish welfare and management practices. This study evaluated the anaesthetic efficacy of Origanum vulgare essential oil (OVEO) in juvenile tambaqui ( Colossoma macropomum ), an important Amazonian species. A total of 108 fish were exposed to immersion baths with increasing OVEO concentrations (19.04–57.12 mg L⁻ 1 ). Behavioral endpoints...

The superorder Xenarthra comprises anteaters, sloths and armadillos, all endemic of the Americas. Despite their distinctive evolutionary history, information regarding viruses affecting this group remains scarce. Herein, we report a gammaherpesvirus infection in a two-toed sloth ( Choloepus didactylus ) under human care that presented recurrent oral vesicular lesions and oral candidiasis. The gammaherpesvirus identified in ora...