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The effect of nitrogen deposition on forest carbon sequestration: a model‐based analysisNARA Subscribed
The perturbation of the global nitrogen (N) cycle due to the increase in N deposition over the last 150 years will likely have important effects on carbon (C) cycling, particularly via impacts on forest C sequestration. To investigate this effect, and the relative importance of different mechanisms involved, we used the Generic Decomposition And Yield (G'DAY) forest C–N cycling model, introducing some new assumptions which foc...
The European carbon balance. Part 3: forestsNARA Subscribed
We present a new synthesis, based on a suite of complementary approaches, of the primary production and carbon sink in forests of the 25 member states of the European Union (EU‐25) during 1990–2005. Upscaled terrestrial observations and model‐based approaches agree within 25% on the mean net primary production (NPP) of forests, i.e. 520±75 g C m −2 yr −1 over a forest area of 1.32 × 10 6 km 2 to 1.55 × 10 6 km 2 (EU‐25). New e...
Expression and characterization of the periplasmic cobalamin‐binding protein of Photobacterium damselae subsp. piscicidaNARA Subscribed
Cobalamin (vitamin B 12 ) is an essential cofactor in a variety of enzymatic reactions and most prokaryotes contain transport systems to import vitamin B 12 . A gene coding for a periplasmic cobalamin‐binding protein of Photobacterium damselae subsp. piscicida was identified by in silico analysis of sequences from a genomic library. The open reading frame was composed of 834 bp encoding a protein of 277 amino acids. The protei...
Development of a multiplex PCR assay for Photobacterium damselae subsp. piscicida identification in fish samplesNARA Subscribed
A multiplex polymerase chain reaction protocol for the detection of Photobacterium damselae and subspecies piscicida and damselae discrimination, with internal amplification control, was developed. Assay specificity was assessed by testing 19 target and 25 non‐target pure cultures. The detection limit was 500 fg, corresponding to 100 genome equivalents. The optimized protocol was also prevalidated with spleen, kidney and blood...
Reliable models are required to assess the impacts of climate change on forest ecosystems. Precise and independent data are essential to assess this accuracy. The flux measurements collected by the EUROFLUX project over a wide range of forest types and climatic regions in Europe allow a critical testing of the process‐based models which were developed in the LTEEF project. The ECOCRAFT project complements this with a wealth of...
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