Fernando Machado Machado results 215
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Wastewater-based surveillance of norovirus GI and GII: Comparative performance of commercial and in-house RT-qPCR AssaysNARA Subscribed
Noroviruses, the leading cause of nonbacterial acute gastroenteritis outbreaks, are widely detected in wastewater worldwide and reflect community-level viral circulation. These RNA viruses exhibit high mutation and recombination rates, favoring the emergence of novel variants with epidemiological relevance. In this study, wastewater-based epidemiology was applied to monitor the circulation of norovirus genogroups GI and GII in...
Moraxella tarda sp. nov., a Novel Species Isolated from Nasal Cavity of Cattle with Infectious Bovine KeratoconjunctivitisNARA Subscribed
A novel bacterial species was isolated from the nasal mucosa of Hereford cattle exhibiting early clinical signs of infectious bovine keratoconjunctivitis on a beef cattle farm in the Pampa biome, southern Brazil, in 2018. Cells were Gram-stain-negative cocci and exhibited slow growth under standard laboratory conditions, with optimal growth at 35 °C, pH 7.5, and 1.0% NaCl. The draft genome comprised approximately 1.86 Mbp (Gen...
Assemblage structure and ecological functions of dung beetles in exotic pastures in the Southwestern AmazonNARA Subscribed
The implementation of exotic pastures in the Amazon causes drastic negative impacts on its biodiversity. Nevertheless, some species with the ability to colonize pastures may provide important ecosystem services for livestock development in tropical pastures, as in the Amazonian biome. In this study, we evaluated the diversity and assemblage structure of dung beetles, as well as measured their ecological functions (i.e., dung r...
Planktonic ciliates in Neotropical streams: taxonomic and numerical sufficiency and implications for environmental impact assessmentNARA Subscribed
Bioindicators are widely used to describe environmental impacts through rapid and cost-effective assessments. However, identifying them at the species level with detailed quantitative sampling is time-consuming, expensive, and requires specialized knowledge. This study evaluated, using planktonic ciliates, taxonomic and numerical sufficiency approaches to test whether higher taxonomic levels and different numerical resolutions...
Random mutagenesis combined with genome-scale analysis represents a powerful approach to uncover metabolic reprogramming in non-model microalgae. In this study we investigated the strain Desmodesmus sp. Petrobras/FURG, a thermotolerant and contamination-resistant microalga with potential for large-scale cultivation. UV mutagenesis followed by iodine-vapor screening yielded the starch-deficient mutant tN-30, which maintained wi...
Integrating fossil data in ecological niche models to improve predictions of future habitat of Caribbean coralsNARA Subscribed
Ecological niche models (ENMs) are used to assess the abiotic preferences of species by linking their occurrences to the environmental conditions in which they live. We developed a fossil‐informed ENM framework that integrates mid‐Holocene and modern occurrences to test niche stability and reconstruct abiotic niche characteristics for four critical reef‐building Caribbean coral species (elkhorn coral [ Acropora palmata ], stag...
The Cerrado biome, spanning ~2 million km 2 , is one of the most extensive and biodiverse tropical savannas, yet it is paradoxically dominated by only 30 hyperdominant tree species (~2% of all species, > 50% of all stems). However, their vulnerability to climate change and the effectiveness of current conservation efforts remain uncertain. By combining (i) species distribution models calibrated with edaphic‐climatic predictors...
Successional agroforestry systems as land use that promote the restoration and conservation of ant assemblages in the AmazonNARA Subscribed
Land-use changes, driven by the expansion of agriculture and livestock farming, are one of the main drivers of biodiversity loss in the Amazon region. In this context, Succesional Agroforestry Systems - SAFs, emerge as a potential alternative to reconcile agricultural production with biodiversity conservation and restoration. This study assessed the impact of different land-use types on ant diversity and the composition of hab...