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Stem Cell Reviews and Reports · 2023 · Vol. 19 · Issue 4 · Springer
Initiation of meiosis is the most difficult aspect of inducing competent oocytes differentiation from human stem cells in vitro . Human induced pluripotent stem cells (hiPSCs) and embryonic stem cells (hESCs) were cultured with follicle fluid, cytokines and small molecule to induced oocyte-like cells (OLCs) formation through a three-step induction procedure. Expression of surface markers and differentiation potential of germ c...