As a critical regulating component in subsea Christmas tree systems, the cage-type choke valve governs the overall production efficiency and operational reliability of offshore oil and gas exploitation owing to its unique internal flow field characteristics. In accordance with the in situ operating conditions of the target oilfield, this paper performs a numerical simulation and systematic analysis on the flow field behaviors...

Using a three-factor orthogonal design, we investigated the transcriptomic and histological responses of triploid rainbow trout (Oncorhynchus mykiss) gills across three salinities (10, 20, and 30 ppt) over 60 days, with temperature and body size as background conditions. Results showed that 20 ppt was associated with extensive transcriptional reprogramming, with 3333 differentially expressed transcripts (DETs), whereas 30 ppt...

Rainbow trout (Oncorhynchus mykiss) is an important cold-water aquaculture species and a tractable salmonid model for functional genomics. Rapid advances in chromosome-level genome assemblies, genetic variation resources, regulatory annotations, tissue and cell models, controlled challenge systems, and genome-editing technologies have established an increasingly integrated framework for linking genomic variation with measurabl...

In suspended mussel farms, culture-layer food supply can be limited, whereas the 4–8 m subsurface layer is rich in particulate organic matter and seston. Using a representative aquaculture block off Gouqi Island, China, this study examined the hydrodynamic and transport effects of non-uniform hanging-depth layouts. The 0° uniform-depth layout was the reference. Five configurations were modeled: V-shaped, inverted V-shaped, uni...

The gravity navigation reference map is a foundational element of gravity matching-aided navigation systems, as it directly determines positioning accuracy and overall navigation performance. To improve fused gravity anomalies and reduce reliance on manually selected parameters, this study proposes an adaptive multi-scale frequency-domain reconstruction (AMFR) algorithm. Specifically, the target ocean covered by multi-source s...

Large-scale fishing bans are increasingly implemented to restore aquatic ecosystems, yet their effects on lower trophic levels remain poorly understood. As one of the world’s largest estuarine systems, the Yangtze River Estuary has been subjected to a ten-year fishing ban since 2021, providing a unique opportunity to potentially examine the response of plankton community to reduced fishing pressure along a steep salinity–nutri...

(1) Background: Vaccination is crucial for Ctenopharyngodon idella, yet the impact of delivery methods on welfare and immunity requires investigation. (2) Methods: This study compared manual (HI-V) and automated (AI-V) injections of inactivated Aeromonas hydrophila vaccine. (3) Results: Both methods induced protective immunity, with AI-V achieving a slightly higher Relative Percent Survival (41.67%) than HI-V (37.84%). AI-V pr...

Deep-sea transportation is faced with complex navigation environments, long voyages, limited emergency response resources, and interacting safety risks. Existing studies have mainly focused on individual risk factors, while the correlations and coupling effects among different factors have received insufficient attention. To identify the key risk factors affecting deep-sea transportation safety, this paper proposes a novel key...

Visceral white spot disease caused by Pseudomonas plecoglossicida poses a severe threat to large yellow croaker (Larimichthys crocea) aquaculture. This study investigated the immunomodulatory effects and underlying mechanisms of Pseudostellaria heterophylla extract against P. plecoglossicida infection in L. crocea. Fish were fed a basal diet supplemented with 1% P. heterophylla extract for 30, 45, and 60 days, followed by intr...

Wave height is a critical parameter for marine hazard warning systems and the structural safety of offshore engineering. The Bohai Sea and its surrounding region is an important economic hub in northern China and serves as the maritime route for the coastal provinces of North, Northwest, and Northeast China. Therefore, the sea state in the Circum-Bohai Sea has a significant impact on the Bohai economic circle. This study analy...

The Chinese bahaba (Bahaba taipingensis), an endangered marine fish, is highly vulnerable to infectious spleen and kidney necrosis virus (ISKNV). In this work, we developed a gill filament-derived cell line, designated BTG, to investigate how these cells respond to ISKNV over time, specifically from 3 to 24 h post-infection (hpi). BTG cells grew steadily, displayed a diploid chromosome number of 2n = 48, demonstrated high tran...

Neolissochilus heterostomus, a cyprinid fish endemic to Yunnan Province, China, is highly valued for both ornamental and edible purposes, yet its wild populations are currently declining and classified as endangered. In this study, we present a chromosome-level genome assembly of N. heterostomus, utilizing the PacBio HiFi and Hi-C strategies. The assembled genome spans 1793.99 Mb and is anchored to 50 chromosomes, comprising a...

The sustainable production of yellow catfish (Pelteobagrus fulvidraco) fry is critical for aquaculture, yet early developmental stages face high mortality and nutritional challenges. This study evaluated the effects of dietary supplementation with broken-cell wall P. rhodozyma on growth performance, organ development, enzyme activities, and gut microbiota composition in yellow catfish fry. Dietary supplementation with broken-c...

To determine the optimal water temperature range for artificial breeding and early development of Osteochilus salsburyi, six water temperature gradients (14, 18, 22, 26, 30, and 34 °C) were set to systematically investigate the effects of water temperature on artificial reproduction efficiency, embryonic development, the morphology of newly hatched larvae, larval growth performance, and the timing of organ development in O. sa...

LNG storage tanks are essential facilities for large-scale storage and transportation of cryogenic energy. Because of the flammable, explosive, and ultra-low-temperature characteristics of liquefied natural gas, failures in such systems may result in serious consequences for operational safety and the surrounding environment. Effective identification and prioritization of potential failure modes are therefore crucial for safe...

The suction caisson foundation has been extensively adopted for offshore wind turbine infrastructure owing to its adaptability to deep-water environments, cost-effectiveness, and convenient construction. However, such foundations suffer from relatively low horizontal and vertical bearing capacities when embedded in soft clay deposits. To address this limitation, this study proposes a novel mobile jet-reinforcement technique an...

Four new species of Chromadorea (Nematoda) from marine benthic habitats in Chinese sea areas are described and illustrated. Halichoanolaimus parvulus sp. nov. is characterized by a comparatively smaller body; amphideal fovea comprising 4.5–5 turns; spicules arched, middle portion broad, progressively attenuating distally; gubernaculum slender, comprising two detached lateral elements becoming narrower toward the distal end; a...

Cylindrical floating production storage and offloading (FPSO) units are advancing into deeper waters. Overcoming the severe challenges posed by complex deepwater environments requires the design of mooring systems that balance economic efficiency and mooring performance. This paper proposes an innovative optimization method for cylindrical FPSO mooring systems, combining the Kolmogorov–Arnold Network (KAN) with the Non-dominat...

Efficient detection of fish diseases is essential for intelligent health monitoring and timely intervention in aquaculture. However, current computer vision models remain computationally intensive, hindering their deployment on resource-constrained edge devices in aquaculture applications. To this end, this study developed a lightweight detection framework based on an improved You Only Look Once (YOLO), aiming to achieve a fav...

Offshore wind power currently shows the trend of a larger wind turbine capacity and deep-sea wind farm sites. Traditional wind farms with single-type wind turbines can hardly accommodate the dual requirements of high-efficiency power generation and wake effect mitigation of wind farms. Moreover, the wind shear of fixed wind turbines and the platform motion of floating wind turbines result in insufficient adaptability to a hybr...

Short-sea Roll-on/Roll-off passenger (Ro-Pax) corridors rely on tightly interconnected port pairs. These corridors face significant challenges in recovering efficiently after major disruptions, as recovery operations are often managed separately by each port, prioritizing the clearance of local backlogs. This can lead to system-wide inefficiencies due to the operational dependencies between ports and strict navigational rules....

Water-soluble pectin (WSP) is a soluble dietary fiber with a high esterification degree and certain viscosity and emulsifying properties. It has diverse bioactivities—including antioxidant, anticancer, and anti-inflammatory properties. This study aimed to investigate the in vitro antioxidant mechanisms of water-soluble pectin, and the in vivo effects of intestinal antioxidant capacity and gut microbiota composition in hybrid g...

This 90-day trial evaluated the integrated benefits of biofloc technology (BFT) in lined ponds for intensive Penaeus vannamei culture, comparing it with a conventional water-exchange (WE) system. The BFT system maintained favorable water quality with a 68.4% reduction in cumulative water exchange. Concentrations of toxic total ammonia–nitrogen (TAN) and nitrite–nitrogen (NO2−-N) were better controlled, and total suspended soli...

The molting of crustaceans is accompanied by exoskeleton reconstruction. To reveal the molecular regulation mechanism of exoskeleton remodeling, the transcriptomic profiles of the exoskeleton across the entire molting process in the red swamp crayfish Procambarus clarkii were investigated by RNA sequencing, yielding a total of 7671 differentially expressed genes (DEGs) across five different molting stages. Notably, the key DEG...

Submarine pipelines serve as the primary transportation infrastructure for offshore energy resources and are becoming increasingly important in modern industrial and domestic applications. A series of laboratory experiments on the dynamic seabed response of three-dimensional pipeline-seabed systems under random wave and current conditions was conducted. The results demonstrate as follows: (1) Under the combined action of rando...