Rapid depressurization and warming during recovery can trigger stress in deep-sea microbes and accelerate RNA degradation. We developed a remotely operated vehicle (ROV)-oriented multi-sequence microbial sampler for 2000 m sampling (20 MPa, 2 °C) that integrates in situ filtration with immediate RNAlater injection (an RNA stabilization reagent), collecting up to 12 samples per dive. A Dirichlet sampling–B-spline–SVM framework...

Accurate timing of myelination is crucial for the proper functioning of the central nervous system. Here, we identified a de novo heterozygous mutation in TMEM63A (c.1894G>A; p. Ala632Thr) in a 7-year-old boy exhibiting hypomyelination. A Ca 2+ influx assay suggested that this is a loss-of-function mutation. To explore how TMEM63A deficiency causes hypomyelination, we generated Tmem63a knockout mice. Genetic deletion of TMEM63...