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Click Chemistry-Based Quantification of Extracellular Matrix Turnover for Drug Screening and Regenerative MedicineNARA Subscribed
Purpose To develop and validate a bioorthogonal labeling approach for quantifying ECM remodeling in living cell and tissue culture systems. Methods Strain-promoted azide-alkyne (SPAAC) reactions, or copper-free click chemistry, were used to fluorescently label newly synthesized glycan and protein matrix components. ECM synthesis and degradation was quantified in cartilage explants, human mesenchymal stem cells, and SKBR3 breas...