Keymessage Standardized microhaplotype databases for eight diverse crops enable multiallelic analyses, comparative genetics, and breeding decisions. Abstract Microhaplotypes are short genomic segments that contain multiple tightly linked variants, providing multi-allelic data that can enhance genetic resolution compared to traditional biallelic single nucleotide polymorphism (SNP) markers. Here, we present the creation and uti...

Engineering crop plants with the biological nitrogen fixation pathway is a longstanding goal of modern agriculture. Dinitrogenase reductase (NifH) is a critical component of the biological nitrogen fixation pathway, with multiple roles in metal cofactor assembly and catalysis. This enzyme must be folded correctly as a soluble homodimer and loaded with the [4Fe-4S] metallocluster for function. Previous studies have found that K...

Herbivores play key roles on reefs by consuming algae that compete with corals. Cleaning mutualisms can alter the distribution and feeding behavior of herbivorous fishes and may indirectly affect benthic communities. These dynamics were examined on patch reefs with and naturally without the bluestreak cleaner wrasse, Labroides dimidiatus , in July 2021 in Moorea, French Polynesia (17°28’35.52"S, 149°48’58.35"W) using herbivory...

Lancefield Group C Streptococcus dysgalactiae recently emerged as a catfish pathogen in the US. Reports from hatcheries suggest S. dysgalactiae causes substantial broodfish loss. We aimed to develop a challenge model and evaluate vaccine efficacy against S. dysgalactiae in juvenile channel catfish ( Ictalurus punctatus ). Three S. dysgalactiae (14–1, 14–24 and 14–25) isolates were tested for virulence. Intracoelomic (IC) injec...

Purpose Many abdominal aortic aneurysm (AAA) patients develop intraluminal thrombus (ILT), but the role of ILT in AAA progression and rupture is poorly understood. Methods To evaluate ILT in AAAs, we induced AAAs in C57Bl6/J mice via surgical application of topical elastase to the abdominal aorta below the renal arteries and administration of β-aminopropionitrile (BAPN) in drinking water. Ultrasound images of these AAAs were c...

At high latitudes, hibernating species must replenish their energy reserves within a 4–6 month active season. During this window, insectivorous bats at higher latitudes must consume sufficient arthropod prey to support reproduction and migration, despite suboptimal weather and prey abundance that varies across time and space. Local declines in body size of endangered little brown bats ( Myotis lucifugus ) suggest ongoing nutri...