Peroxisomes are ubiquitous, oxidative organelles in eukaryotes with conserved functions in cellular lipid metabolism and redox balance. They are now recognised as key metabolic organelles of medical importance, with contributions to antiviral signalling, pathogen defence, innate immunity, cancer and neurodegeneration. Our current knowledge of peroxisomes and the molecular understanding of their diverse functions and roles in d...

Organelle proteomics is a valuable approach to identify novel proteins in a confined cellular compartment thereby increasing our understanding of the function of individual organelles in cellular metabolism or signaling. A major obstacle in annotating organelle proteomes by mass spectrometry(MS)-based proteomics is to distinguish between true organelle constituents and contaminants co-purified during fractionation. Here we giv...

Peroxisomes are highly dynamic, oxidative organelles with key metabolic functions in cellular lipid metabolism, such as the β-oxidation of fatty acids and the synthesis of myelin sheath lipids, as well as the regulation of cellular redox balance. Loss of peroxisomal functions causes severe metabolic disorders in humans. Furthermore, peroxisomes also fulfil protective roles in pathogen and viral defence and immunity, highlighti...