Pathogen identification is key to the management of periprosthetic joint infection (PJI). Staphylococcus epidermidis is a major cause of PJI. Differentiating between invasive and contaminating S. epidermidis from joint specimens is challenging, and usually S. epidermidis is only considered a true invasive isolate when grown from two or more independent tissue samples. Thus, the detection of S. epidermidis in single synovial fl...

Neurons in central nervous systems receive multiple synaptic inputs and transform them into a largely standardized output to their target cells—the action potential. A simplified model posits that synaptic signals are integrated by linear summation and passive propagation towards the axon initial segment, where the threshold for spike generation is either crossed or not. However, multiple lines of research during past decades...

Carbon, capture, and storage (CCS) is an important bridging technology to combat climate change in the transition toward net-zero. The FluidFlower concept has been developed to visualize and study CO 2 flow and storage mechanisms in sedimentary systems in a laboratory setting. Meter-scale multiphase flow in two geological geometries, including normal faults with and without smearing, is studied. The experimental protocols deve...

Understanding porous media flow is inherently a multi-scale challenge, where at the core lies the aggregation of pore-level processes to a continuum, or Darcy-scale, description. This challenge is directly mirrored in image processing, where pore-scale grains and interfaces may be clearly visible in the image, yet continuous Darcy-scale parameters may be what are desirable to quantify. Classical image processing is poorly adap...

We present a framework for integrated experiments and simulations of tracer transport in heterogeneous porous media using digital twin technology. The physical asset in our setup is a meter-scale FluidFlower rig. The digital twin consists of a traditional physics-based forward simulation tool and a correction technique which compensates for mismatches between simulation results and observations. The latter augments the range o...

Successful deployment of geological carbon storage (GCS) requires an extensive use of reservoir simulators for screening, ranking and optimization of storage sites. However, the time scales of GCS are such that no sufficient long-term data is available yet to validate the simulators against. As a consequence, there is currently no solid basis for assessing the quality with which the dynamics of large-scale GCS operations can b...

Increasing worldwide, prevalence of carbapenem-resistant gram-negative bacteria demands urgent a need for rapid detection and accurate identification of carbapenemases. The BD Phoenix CPO detect (PCD) assay possesses an in-built capacity for parallel susceptibility testing and detection of carbapenemases. Here, the ability of the assay to detect and classify carbapenemase production was tested in a collection of carbapenem-res...

Logging, pervasive across the lowland tropics, affects millions of hectares of forest, yet its influence on nutrient cycling remains poorly understood. One hypothesis is that logging influences phosphorus (P) cycling, because this scarce nutrient is removed in extracted timber and eroded soil, leading to shifts in ecosystem functioning and community composition. However, testing this is challenging because P varies within land...