Background and objective Histamine is the principal effector of anaphylaxis, and circulating levels correlate well with symptom severity in Hymenoptera venom–induced anaphylaxis. Adrenaline, the recommended first-line therapy, does not neutralize histamine and may cause relevant adverse effects. Recombinant human diamine oxidase (rhDAO), engineered with a mutated heparin-binding motif, exhibits improved pharmacokinetics and ra...

Fosfomycin (FOF) exhibits broad-spectrum antimicrobial activity, and is mainly used in combination therapy. Previous in vitro studies have shown synergistic effects of FOF in combination with flucloxacillin (FLX) against Staphylococcus aureus isolates. This study aims to validate these findings in vitro and investigate the synergistic effect in an in vivo Galleria mellonella model. Five methicillin- and FOF-susceptible isolate...

Objective and design SARS-CoV-2 vaccines are recommended for mastocytosis patients. We describe clinical symptoms, chemokine, cytokine, metabolomic and lipidomic derangements in a systemic mastocytosis patient following mRNA-1273 booster vaccination. Methods Twenty-eight chemokines and cytokines, 41 amino acids and 16 lipid classes were quantified with state-of-the-art methods. Results Mast cell activation (MCA) symptoms start...

Objective To test whether recombinant human diamine oxidase (rhDAO) with a mutated heparin-binding motif (mHBM), which shows an increased alpha-distribution half-life, prevents histamine-induced hemodynamic effects. Material Thirty-eight female guinea pigs were either pretreated with rhDOA_mHBM or buffer. Treatment and methods Guinea pigs received a continuous infusion of histamine. Heart rate (HR), body core temperature and m...

Mesenchymal stromal/stem cells (MSCs) have great capacity for immune regulation. MSCs provide protective paracrine effects, which are partially exerted by extracellular vesicles (EVs). It has been reported that MSCs-derived EVs (MSC-EVs) contain soluble factors, such as cytokines, chemokines, growth factors and even microRNAs, which confer them similar anti-inflammatory and regenerative effects to MSCs. Moreover, MSCs modulate...

Implementing education for sustainable development (ESD) in higher education institutions (HEIs) is critical to facilitating a transition toward sustainable development. However, little is known about the specific implementation processes that lead to the institutionalization of sustainability curricula in HEIs. This meta-study and cluster analysis uses 131 international case studies to shed light on six distinct implementatio...

The Caspian Sea level (CSL) has undergone variations of more than 3 m during the past century with important implications for the life of coastal people, economy and the ecosystem. The origin of these variations as well as future changes in the Caspian water budget are still a matter of debate. Here, the major modes of North Atlantic winter climate variability and atmospheric teleconnections that have a potential effect on the...

Summary Pseudomonas aeruginosa is an environmental microorganism and a causative agent of diverse acute and chronic, biofilm‐associated infections. Advancing research‐based knowledge on its adaptation to conditions within the human host is bound to reveal novel strategies and targets for therapeutic intervention. Here, we investigated the traits that P. aeruginosa PA14 as well as a virulence attenuated Δ lasR mutant need to su...